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avidin biotin peroxidase complex solution  (Vector Laboratories)


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    Vector Laboratories avidin biotin peroxidase complex solution
    Avidin Biotin Peroxidase Complex Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 16815 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abc-solution+abc-kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Universal)/pm42892892-383-17-25
    Average 96 stars, based on 16815 article reviews
    avidin biotin peroxidase complex solution - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: β-Cyclodextrin-Complexed 1,8-Cineole Attenuates Motor Deficits and Preserves Tyrosine Hydroxylase Immunoreactivity in a Reserpine-Induced Mouse Model of Parkinsonism
    Article Snippet: After primary antibody incubation, the sections were washed and incubated for 2 h with a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, USA) diluted in Triton X-100 solution. .. Sections were subsequently incubated for an additional 2 h with an avidin-biotin-peroxidase complex solution (Vectastain Elite ABC Kit, Vector Laboratories, USA). .. Immunoreactivity was visualized using 3,3′-diaminobenzidine (DAB; Sigma-Aldrich, USA) in the presence of 0.01% H2O2 for 2 min in TH-stained sections and for 1 min in α-Syn-stained sections.

    Article Title: KDM6 Histone Demethylases Tune Pancreatic Cancer Plasticity
    Article Snippet: For 131 immunofluorescence, slides were mounted with DAPI (Thermo Fisher, P36981). .. For 132 immunohistochemistry, sections were further incubated for 30 min in ABC solution (Vector 133 Laboratories, PK-6100), developed using the DAB Substrate Kit (Vector Laboratories, SK-4100) 134 as per manufacturer’s instructions and counterstained with hematoxylin. .. For human tissues, IHC 135 for Notch1, Jagged1 and KDM6B was performed using a Bond RXm system with pre-treatment 136 with Epitope Retrieval Solution 2 (Leica Biosystems); IHC for Kdm6a was performed on a 137 Benchmark XT (RRID: SCR_026335) system with pre-treatment with Cell Conditioning Solution 138 2 (Ventana Medical Systems).

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The following day, the sections were washed in 0.05 M TBS before being incubated with the biotinylated goat anti-rabbit (1:300, catalog: 111-066-046, Jackson ImmunoResearch) for 2 h at RT, and washed again in 0.05 M TBS. .. The sections were incubated for 1 h in an ABC solution (1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories), washed in TBS, and the staining was revealed with a DAB incubation, and washed in PB as described above. ..

    Article Title: Calcium-binding protein expression cannot serve as a general classifier for GABAergic neurons in macaque cortex
    Article Snippet: .. After this incubation and rinsing (3 × 5 minutes), sections were incubated for 1 hour in an avidin-horseradish peroxidase solution (Vectastain Elite ABC kit, Vector Laboratories Cat# PK-6100, RRID:AB_2336819) and rinsed again in PBS (3 × 5 minutes). .. Antigenic sites were visualized using Vector’s VIP Substrate kit (Vector Laboratories Cat# SK-4600, RRID:AB_2336848), with reaction time controlled by eye (range: 3–11 minutes).

    Article Title: Canonical Wnt induction by OTULIN prevents keratinocyte death and skin inflammation
    Article Snippet: Next, the slides were incubated with biotin-coupled goat anti-rabbit (1:500, Dako). .. Subsequently, slides were incubated in ABC solution (Vectastain ELITE ABC Kit Standard, Vector Laboratories). .. Peroxidase activity was detected by adding diaminobutyric acid (DAB) substrate [ImmPACT DAB Peroxidase (HRP) Substrate, Vector Laboratories].

    Avidin-Biotin Assay:

    Article Title: β-Cyclodextrin-Complexed 1,8-Cineole Attenuates Motor Deficits and Preserves Tyrosine Hydroxylase Immunoreactivity in a Reserpine-Induced Mouse Model of Parkinsonism
    Article Snippet: After primary antibody incubation, the sections were washed and incubated for 2 h with a biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, USA) diluted in Triton X-100 solution. .. Sections were subsequently incubated for an additional 2 h with an avidin-biotin-peroxidase complex solution (Vectastain Elite ABC Kit, Vector Laboratories, USA). .. Immunoreactivity was visualized using 3,3′-diaminobenzidine (DAB; Sigma-Aldrich, USA) in the presence of 0.01% H2O2 for 2 min in TH-stained sections and for 1 min in α-Syn-stained sections.

    Article Title: Calcium-binding protein expression cannot serve as a general classifier for GABAergic neurons in macaque cortex
    Article Snippet: .. After this incubation and rinsing (3 × 5 minutes), sections were incubated for 1 hour in an avidin-horseradish peroxidase solution (Vectastain Elite ABC kit, Vector Laboratories Cat# PK-6100, RRID:AB_2336819) and rinsed again in PBS (3 × 5 minutes). .. Antigenic sites were visualized using Vector’s VIP Substrate kit (Vector Laboratories Cat# SK-4600, RRID:AB_2336848), with reaction time controlled by eye (range: 3–11 minutes).

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa.
    Article Snippet: .. Detection was achieved using a biotinylated secondary antibody diluted in TBS, followed by incubating the slides with avidin-biotin-peroxidase complex solution (ABC, Vectastain Elite ABC Kit; Vector Laboratories, USA) each for 30 minutes at RT and exposure to a 3-amino-9-ethylcarbazole substrate (AEC substrate Kit, Abcam, UK). .. For PAX5 an ImmPRESS Polymer Kit (Vector Laboratories, USA) was used, followed by AEC substrate.

    Immunohistochemistry:

    Article Title: KDM6 Histone Demethylases Tune Pancreatic Cancer Plasticity
    Article Snippet: For 131 immunofluorescence, slides were mounted with DAPI (Thermo Fisher, P36981). .. For 132 immunohistochemistry, sections were further incubated for 30 min in ABC solution (Vector 133 Laboratories, PK-6100), developed using the DAB Substrate Kit (Vector Laboratories, SK-4100) 134 as per manufacturer’s instructions and counterstained with hematoxylin. .. For human tissues, IHC 135 for Notch1, Jagged1 and KDM6B was performed using a Bond RXm system with pre-treatment 136 with Epitope Retrieval Solution 2 (Leica Biosystems); IHC for Kdm6a was performed on a 137 Benchmark XT (RRID: SCR_026335) system with pre-treatment with Cell Conditioning Solution 138 2 (Ventana Medical Systems).

    Plasmid Preparation:

    Article Title: KDM6 Histone Demethylases Tune Pancreatic Cancer Plasticity
    Article Snippet: For 131 immunofluorescence, slides were mounted with DAPI (Thermo Fisher, P36981). .. For 132 immunohistochemistry, sections were further incubated for 30 min in ABC solution (Vector 133 Laboratories, PK-6100), developed using the DAB Substrate Kit (Vector Laboratories, SK-4100) 134 as per manufacturer’s instructions and counterstained with hematoxylin. .. For human tissues, IHC 135 for Notch1, Jagged1 and KDM6B was performed using a Bond RXm system with pre-treatment 136 with Epitope Retrieval Solution 2 (Leica Biosystems); IHC for Kdm6a was performed on a 137 Benchmark XT (RRID: SCR_026335) system with pre-treatment with Cell Conditioning Solution 138 2 (Ventana Medical Systems).

    Transcranial Magnetic Stimulation:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The following day, the sections were washed in 0.05 M TBS before being incubated with the biotinylated goat anti-rabbit (1:300, catalog: 111-066-046, Jackson ImmunoResearch) for 2 h at RT, and washed again in 0.05 M TBS. .. The sections were incubated for 1 h in an ABC solution (1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories), washed in TBS, and the staining was revealed with a DAB incubation, and washed in PB as described above. ..

    Article Title: A multimodal in situ framework for spatial characterization applied on SARS-CoV-2 live-attenuated vaccines in the nasal mucosa.
    Article Snippet: .. Detection was achieved using a biotinylated secondary antibody diluted in TBS, followed by incubating the slides with avidin-biotin-peroxidase complex solution (ABC, Vectastain Elite ABC Kit; Vector Laboratories, USA) each for 30 minutes at RT and exposure to a 3-amino-9-ethylcarbazole substrate (AEC substrate Kit, Abcam, UK). .. For PAX5 an ImmPRESS Polymer Kit (Vector Laboratories, USA) was used, followed by AEC substrate.

    Staining:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The following day, the sections were washed in 0.05 M TBS before being incubated with the biotinylated goat anti-rabbit (1:300, catalog: 111-066-046, Jackson ImmunoResearch) for 2 h at RT, and washed again in 0.05 M TBS. .. The sections were incubated for 1 h in an ABC solution (1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories), washed in TBS, and the staining was revealed with a DAB incubation, and washed in PB as described above. ..



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