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abc solution  (Vector Laboratories)


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    Structured Review

    Vector Laboratories abc solution
    Abc Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12838 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/abc-solution+abc-kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc12996694-165-9-17
    Average 99 stars, based on 12838 article reviews
    abc solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death
    Article Snippet: Developing solution from the EdU Click-it Kit (Thermo Fisher; Ref No. C10269; biotin azide antibody at 1:800) (component amounts published in online protocol) was then made and applied to slides which were incubated for 2-3 hours at room temperature or overnight at 4C. .. Solution was then thoroughly rinsed and avidin-biotin peroxidase (ABC) complex (Vector Laboratories; SKU PK-6100) was added for signal amplification as per manufacturer’s protocol. ..

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
    Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories, USA) for 2 hours. .. AR TI CL E IN P RE SS ARTICLE IN PRESS This was followed by three rinses in PBS, after which the sections were developed in a solution containing the chromogen (NovaRED substrate kit, peroxidase; SK-4800, Vector Laboratories, USA) according to the manufacturer’s instructions.

    Article Title: Motor sequence analysis as a sensitive biomarker of dopaminergic degeneration in a non-human primate model of parkinsonism
    Article Snippet: .. After three more washes, the sections were incubated in an avidin-biotin enzyme complex solution (ABC kit, PK-4000, Vector, Burlingame, CA) for 1 hour. .. Then, they were rewashed and subjected to the peroxidase detection reaction using DAB (diaminobenzidine – Sigma-Aldrich, Inc) as the chromogen.

    Article Title: Targeting integrin αvβ3 by chimeric antigen receptor neural stem cell (CAR-NSC) therapy for stroke
    Article Snippet: .. Sections were then treated with the Avidin-Biotin-Complex (ABC) kit solution (Vector Laboratories, #PK-6100) according to the manufacturer’s protocol. ..

    Amplification:

    Article Title: Precursor Cells in the Parenchyma Act in Concert with Ventricular Neural Progenitors to Facilitate Dramatic Astrocyte Turnover and Recovery Following Natural Neuronal Death
    Article Snippet: Developing solution from the EdU Click-it Kit (Thermo Fisher; Ref No. C10269; biotin azide antibody at 1:800) (component amounts published in online protocol) was then made and applied to slides which were incubated for 2-3 hours at room temperature or overnight at 4C. .. Solution was then thoroughly rinsed and avidin-biotin peroxidase (ABC) complex (Vector Laboratories; SKU PK-6100) was added for signal amplification as per manufacturer’s protocol. ..

    Incubation:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The following day, the sections were washed in 0.05 M TBS before being incubated with the biotinylated goat anti-rabbit (1:300, catalog: 111-066-046, Jackson ImmunoResearch) for 2 h at RT, and washed again in 0.05 M TBS. .. The sections were incubated for 1 h in an ABC solution (1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories), washed in TBS, and the staining was revealed with a DAB incubation, and washed in PB as described above. ..

    Article Title: Developmental trajectories of somatostatin-positive interneurons in the human auditory cortex from migration to maturation.
    Article Snippet: The sections were again rinsed thrice in PBS and incubated for 2 hours in a secondary antibody solution (Biotinylated anti-rat antibody raised in goat; 1:250 dilution, BA-9400, Vector Laboratories, Burlingame, CA, USA) at RT. .. After this step, sections were rinsed in PBS three times and incubated in a solution containing the avidin-biotin complex (ABC reagent; PK-6100, Vectastain Elite ABC HRP kit, Vector Laboratories, USA) for 2 hours. .. AR TI CL E IN P RE SS ARTICLE IN PRESS This was followed by three rinses in PBS, after which the sections were developed in a solution containing the chromogen (NovaRED substrate kit, peroxidase; SK-4800, Vector Laboratories, USA) according to the manufacturer’s instructions.

    Article Title: Motor sequence analysis as a sensitive biomarker of dopaminergic degeneration in a non-human primate model of parkinsonism
    Article Snippet: .. After three more washes, the sections were incubated in an avidin-biotin enzyme complex solution (ABC kit, PK-4000, Vector, Burlingame, CA) for 1 hour. .. Then, they were rewashed and subjected to the peroxidase detection reaction using DAB (diaminobenzidine – Sigma-Aldrich, Inc) as the chromogen.

    Staining:

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The next day, the tissues were washed in TBS before being incubated with a goat anti-rabbit biotinylated secondary antibody (1:300, catalog: 111-066-046, Jackson ImmunoResearch) in TBS for 1.5 h at RT, and washed again in TBS. .. The tissues were incubated in an avidin-biotin-complex solution (ABC; 1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories) in TBS for 1 h. The tissues were then washed in TBS and the staining was revealed using incubation with 0.05% of 3,3ʹ diaminobenzidine (DAB) in Tris buffer (TB, 50 mM, pH 8.0) with 0.015% H 2 O 2 for 5 min. ..

    Article Title: Microglial response to sleep deprivation depends on the hippocampal region and paradigm used in adult male mice
    Article Snippet: The following day, the sections were washed in 0.05 M TBS before being incubated with the biotinylated goat anti-rabbit (1:300, catalog: 111-066-046, Jackson ImmunoResearch) for 2 h at RT, and washed again in 0.05 M TBS. .. The sections were incubated for 1 h in an ABC solution (1:100 A; 1:100 B, catalog: PK-6100, Vector Laboratories), washed in TBS, and the staining was revealed with a DAB incubation, and washed in PB as described above. ..



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